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项目的背景及主要用途: 真菌疏水蛋白是由丝状真菌在生长的特定时期分泌的一类具有特殊理化性质的分泌型的小分子量、疏水性蛋白质,它们可以通过自我装配在两相界面形成两亲性蛋白膜,改变介质表面的亲水性和疏水性,是已知表面活性最高的蛋白之一,有着很高的理论价值和应用价值。
真菌疏水蛋白是纯天然生物提取制品,无毒害,无污染;耐酸碱,抗相变能力强。自我装配形成有活性的蛋白膜,具有良好的热稳定性和透气不透水性。由于它的特性,使得它具有众多优点:
(1)自动成膜,使用便利;
(2)透气性优良;
(3)纯天然无化学添加成分;
(4)组织相容性好,避免了严重的排异反应;
(5)耐高温(100摄氏度仍保持活性),易于消毒;
(6)稳定不降解,便于产品的长期保存;
(7)用表面活性剂就可以很容易地清洗;
(8)延展性好,1毫克的成膜活性蛋白在液面就可以展开1平方米的薄膜;
(9)大规模生物发酵,高产,性价比高。
疏水蛋白可广泛应用于生物医学检测(生物芯片、生物传感器)和生物医药(组织工程、器官移植、药物缓释)等多个领域而且具有极为广阔的前景。特别是在临床的烧伤、创伤的创面保护及缓和机体的排异方面拥有极佳的应用前景和发展潜力,为临床病人创面保护和恢复提供了一种安全无毒、操作简便、高效低耗的新手段。
项目技术水平:
已顺利完成了膜表面生物活性真菌疏水蛋白的中试研发,对自主研发的SDS-ATPS抽提方法进行了优化改良,适应于工业化的抽提规模,并优化了疏水蛋白的纯化工艺,使疏水蛋白的总回收率提高至60%以上。
在国际上首先建立了食用真菌灰树花的cDNA文库,克隆到具有自主知识产权的灰树花疏水蛋白基因hgf1(专利申请号200610013116.4)。已经将真菌疏水蛋白应用于生物传感器和生物芯片的固定化。通过对传感器表面修饰、分子间相互作用、纳米材料等领域的研究,实现了分子水平上对分子膜的可控累积和特异选择性识别。研究成果处于国际先进水平。 本项目研究获得多项省部级科技计划资助。
产业化:
1) 20吨发酵罐发酵生产真菌疏水蛋白条件的进一步的优化,按照应用的不同需求制备不同纯度级别的真菌疏水蛋白制剂,应用于病人创面保护和恢复的生物敷料,缓和机体排异性/组织工程/药物缓释等方面的生物制剂,生物传感器和生物芯片的生物活性纳米材料。
2) 通过基因工程的手段,将真菌疏水蛋白与抗体进行融合表达,融合蛋白应用于免疫传感器,将会对生物传感器的特异选择性、稳定性和灵敏度产生数量级程度的提高,在医药卫生领域具有重大意义。
应用价值与发展前景: 真菌疏水蛋白具有自我装配成膜的性质,因此:
(1)可作为蛋白和细胞固定化的媒介,可用于生物传感器和生物芯片,作为引发层,交联上配体或形成融合蛋白,能使特定分子固定化到特定表面。
(2)它能改变表面的属性,保护表面。可用于提高医学器官移植物生物相容性和防止微生物细胞粘附;可应用于医药行业中烧伤、创伤的创面保护,为临床病人创面保护和恢复提供一种安全无毒、操作简便、高效低耗的新手段。
(3)作为一种新型的纳米材料,在药物缓释领域可以发挥重要的作用。纯天然的具有自组装功能的HGFI疏水蛋白提纯于可食用的药物真菌,其生物相容性非常好,用作新型的药物缓释材料,更为安全可靠。疏水蛋白HGFI可通过各种方法行使缓释剂的功能,例如包埋法、微球法、嫁接-共混法等,用途非常广泛,并可以通过对HGFI疏水蛋白的改性来更好的行使缓释剂的功能。   
目前,国际上尚未实现膜活性疏水蛋白的工业化生产,其相关应用产品的开发更为滞后。我们在已实现成膜活性蛋白中试研发和技术和工艺现居国际领先地位的基础上,扩大发酵规模,进行后续产品的开发,无疑会占有宝贵的先机。

为维护中技网平台交易秩序,保护技术需求方合法权益,本人(本单位)特做出以下承诺和声明:
一、本人(本单位)专利技术是经国家知识产权局审定认可,并取得了该项知识产权的专利证书,信息真实,技术可靠,无知识产权纠纷。
二、本人(本单位)愿意根据《中华人民共和国合同法》、《中华人民共和国专利法》、《中华人民共和国知识产权保护法》和《中华人民共和国促进科技成果转化法》以及中技网平台制定的技术转移交易规则和制度进行规范交易,自觉维护技术转移网络交易秩序。
三、如本人(本单位)在技术转移转化过程中有严重违规违约行为,给平台和交易相对方造成经济或财产损失,本人(本单位)愿意承担相应责任。

For maintenance or web platform trading order, protect the legal rights of the technical demand (units), make the following commitment and declaration:

1I (unit) patent technology is approved by the state intellectual property office and obtained the patent certificate of this intellectual property, the information is true, the technology is reliable, and there is no intellectual property dispute.

2, I (unit) willing to according to the law of the People's Republic of China contract law ", "patent law of the People's Republic of China, the intellectual property rights of the People's Republic of China protects a law" and "promote the conversion of scientific and technological achievements of the People's Republic of China" and wowed net platform to develop the trading rules and the system of technology transfer of regulate transactions, consciously safeguard the order of the technology transfer network transactions.

3.If I (unit) in the process of the transformation of technology transfer has seriously violated the breach, to economic or property damage caused by platform and trade each other, I (the unit) are willing to bear the corresponding legal responsibility.

交易规则说明

1、转让方在委托服务商转让期间,首先要认真阅读中技网会员协议和技术商品交易规则,其次要了解中技网有关收费标准,清楚应当遵守的相关权利和义务

2、购买方在中技网平台上认定购买某一项技术商品前,需要按照规定向中技网平台支付定金(完成意向性购买),但知识产权归属以及何时能够使用该项知识产权,视买卖双方协商确定后才能够使用,在此之前,建议您不要使用或侵犯他人的知识产权。

3、本网站对上传在首页各版块展示出售的技术产品实行有偿服务原则,对拟出售的专利技术产品交易价格以及市场前景,中技网将根据财政部无形资产评估有关标准和规定给予客观、公正的评价,具体收费标准请咨询客服人员。

1, the assignor in commissioned services during the transfer, the first thing to read or member agreement and the technical commodity trading rules, second to understand relevant charge standard or network platform, which shall comply with the relevant rights and obligations.

2, the purchaser on or network platform, decided to buy a certain technology products before, need to pay a deposit in accordance with the relevant provisions to or net platform (complete intentional purchase), but the intellectual property ownership and when to be able to use the intellectual property rights, after the negotiation between the buyers and sellers can use, before that, it is recommended that you do not use or infringe others' intellectual property.

3, this website to upload on the home page of each section shows the sale of products shall be paid for the service principle, to plans to sell the patent technology of the product market price and the market prospects, or network will according to the ministry of finance of intangible assets evaluation of relevant standards and regulations to give an objective and fair evaluation, specific charge standard, please consult customer service staff.

交易流程

根据中技网交易规则,技术买卖双方应当遵循友好、自愿和有偿原则进行交易,为此,请技术买卖双方应当遵循中技网制定的交易流程进行有序买卖。
一、买方确定购买意向→支付定金→签订意向性购买协议→买卖双方签订正式协议→买方付款 →办理技术转移交割手续→资金结算→交易完成→退还定金。

二、卖方提交信息资料→上传有效证明文件→平台审核→与平台签订相关协议和信用保证金协议→缴纳信用保证金→平台发布出让信息→确定买家→签订协议→出让技术→资金结算→交易完成→退还信用保证金。

    According to trading rules or network technology between buyer and seller shall follow the principle of friendly, voluntary and paid, for this, please technology between buyer and seller shall follow the wowed the orderly sale network of trading process.

One, the buyer to determine the purchase intentions to pay the deposit, intentional purchase agreement to sign a formal agreement between the seller and the buyer, the buyer's payment to delivery technology transfer formalities, fund settlement - deal - return deposit.

Second, the seller submit information to upload valid documents to review platform, and platform signed the relevant agreement and credit margin agreements to pay credit deposit, platform release transfer information to determine the buyer, signed an agreement to transfer technology, funds and settlements - deal - the refund credit deposit

重要提示

1、技术转让方请务必遵守《中华人民共和国合同法》、《中华人民共和国专利法》、《中华人民共和国知识产权法》、《中华人民共和国促进科技成果转化法》等相关法律法规,诚实守信,不得伪造或提供虚假技术信息。

2、技术购买方在决定购买技术商品前,首先要了解中技网的有关交易流程和技术转让方的详细信息,在购买过程中对有关技术信息产生疑问或不清楚的地方,请随时与技术信息发布单位取得联系。

3、中技网作为市场化运作的专业技术买卖服务平台,由中国技术市场协会主办,是国内权威的专业技术交易分类信息网,专业、专注、权威,面向国内外发布技术转让和技术需求项目信息,服务周到,信誉第一,交易规范,请客户认准中技网官方网址,切勿上当受骗。

1, technology transferor please be sure to abide by the law of the People's Republic of China contract law ", "patent law of the People's Republic of China" and "intellectual property law of the People's Republic of China", "promote the conversion of scientific and technological achievements of the People's Republic of China" and other relevant laws and regulations, the honest code of honor, are not allowed to fake or providing false information technology.

2 the purchaser prior to the decision to buy technology goods, technology, the first thing to understand the assignor or network related transaction process and technology of detailed information, in the process of buying to doubt the related technical information or unclear, please feel free to get in touch with technical information release unit.

3, or as a market-oriented operation of professional technology trading service platform, sponsored by the China technology market association, is the domestic authority of professional technology trading classified information network, professional, dedicated, authority, and the release technology transfer and technology at home and abroad demand project information, service, credit first, trade specification, please customers look or net official web site, please do not be deceived.

郑重声明

  会员在服务期内私自线下与相对交易方进行交易,由当事人自行承担法律后果,所产生的经济或知识产权纠纷中技网不承担任何法律责任。

Membership in the service period to offline, transacting business with relatively by the parties to bear the legal consequences, economic or intellectual property rights disputes or generated by the network does not assume any legal liability.

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