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内容简介:
本课题组已经从黑龙江省长期施用长残效除草剂的土壤中驯化分离出四类除草剂阿特拉津、氟磺胺草醚、甲氧咪草烟和氯嘧磺隆的高效降解菌株5株,利用高效液相色谱法对除草剂的残留量进行测定,完成了菌株的鉴定,研究得到菌株的最适生长条件,已经完成菌剂制作工艺摸索,通过敏感作物的盆栽生测试验,验证了菌株对除草剂污染土壤具有较好的修复能力。另外,对两个玉米品种(克单8号、绥玉10号)的内生细菌进行分离纯化,分离得到的125株内生细菌,并进行了详细的形态及生理生化鉴定。
从中筛选到三株具有促进玉米生长作用的促生菌株,经该菌株发酵液处理的玉米种子及苗期玉米在提升各项生物量指标上与同品种空白对照相比差异显著。对其中效果最好的一株进行分子鉴定,确定其属于芽孢杆菌属的成员Bacillus sp.,并进行了此菌株的发酵条件优化,确定了最佳发酵条件,为后续中试提供了理论依据。
基于以上事实,解毒促生复合菌肥的研究有了很好的基础,本研究的主要内容是构建多菌复合体系,研究各菌株之间的相互作用,并开发一种简便高效的固体菌肥制作方法,测定菌剂的质量指标和其对除草剂的降解效力,通过田间试验研究复合菌肥对农作物的影响,通过HPLC测定土壤中除草剂的降解率,通过作物的生物量来验证其修复效果和促生作用。
目前已申请的专利如下:
1) 一种含有普鲁兰多糖的苏云金芽孢杆菌可湿性粉剂及其制备方法. 中华人民共和国国家知识产权局,专利申请号:201010549039.0
2)一株高效降解氟磺胺草醚的门多萨假单胞菌菌株. 中华人民共和国国家知识产权局,专利申请号:201110031445.2
3) 一株高效降解氟磺胺草醚的福氏志贺氏菌菌株. 中华人民共和国国家知识产权局,专利申请号:201110047793.9
4) 一株高效降解甲氧咪草烟的鲍曼不动杆菌菌株. 中华人民共和国国家知识产权局,专利申请号:201110145022.3
技术指标:
微生物复合菌肥具有促进玉米生长作用的促生菌株,经该菌株发酵液处理的玉米种子及苗期玉米在提升各项生物量指标上与同品种空白对照相比差异显著。
经济效益和社会效益预测:
本项目的成果高效复合菌肥研制成功后,定会为黑龙江省的农业生产提供有利的帮助,能够很好地进行长残效除草剂污染土壤的生物修复,能够更好地促进农作物生长,能够减少我省农业生产的经济损失并且提高农作物的产量,从而获得更高的经济效益,因此解毒和促生高效复合菌肥的研制具有广阔的应用前景。
转让、合作方式和生产条件:采取技术开发的方式实施。本项目要求企业能够进行大规模菌株的发酵生产并进行田间机械化施用作业。

为维护中技网平台交易秩序,保护技术需求方合法权益,本人(本单位)特做出以下承诺和声明:
一、本人(本单位)专利技术是经国家知识产权局审定认可,并取得了该项知识产权的专利证书,信息真实,技术可靠,无知识产权纠纷。
二、本人(本单位)愿意根据《中华人民共和国合同法》、《中华人民共和国专利法》、《中华人民共和国知识产权保护法》和《中华人民共和国促进科技成果转化法》以及中技网平台制定的技术转移交易规则和制度进行规范交易,自觉维护技术转移网络交易秩序。
三、如本人(本单位)在技术转移转化过程中有严重违规违约行为,给平台和交易相对方造成经济或财产损失,本人(本单位)愿意承担相应责任。

For maintenance or web platform trading order, protect the legal rights of the technical demand (units), make the following commitment and declaration:

1I (unit) patent technology is approved by the state intellectual property office and obtained the patent certificate of this intellectual property, the information is true, the technology is reliable, and there is no intellectual property dispute.

2, I (unit) willing to according to the law of the People's Republic of China contract law ", "patent law of the People's Republic of China, the intellectual property rights of the People's Republic of China protects a law" and "promote the conversion of scientific and technological achievements of the People's Republic of China" and wowed net platform to develop the trading rules and the system of technology transfer of regulate transactions, consciously safeguard the order of the technology transfer network transactions.

3.If I (unit) in the process of the transformation of technology transfer has seriously violated the breach, to economic or property damage caused by platform and trade each other, I (the unit) are willing to bear the corresponding legal responsibility.

交易规则说明

1、转让方在委托服务商转让期间,首先要认真阅读中技网会员协议和技术商品交易规则,其次要了解中技网有关收费标准,清楚应当遵守的相关权利和义务

2、购买方在中技网平台上认定购买某一项技术商品前,需要按照规定向中技网平台支付定金(完成意向性购买),但知识产权归属以及何时能够使用该项知识产权,视买卖双方协商确定后才能够使用,在此之前,建议您不要使用或侵犯他人的知识产权。

3、本网站对上传在首页各版块展示出售的技术产品实行有偿服务原则,对拟出售的专利技术产品交易价格以及市场前景,中技网将根据财政部无形资产评估有关标准和规定给予客观、公正的评价,具体收费标准请咨询客服人员。

1, the assignor in commissioned services during the transfer, the first thing to read or member agreement and the technical commodity trading rules, second to understand relevant charge standard or network platform, which shall comply with the relevant rights and obligations.

2, the purchaser on or network platform, decided to buy a certain technology products before, need to pay a deposit in accordance with the relevant provisions to or net platform (complete intentional purchase), but the intellectual property ownership and when to be able to use the intellectual property rights, after the negotiation between the buyers and sellers can use, before that, it is recommended that you do not use or infringe others' intellectual property.

3, this website to upload on the home page of each section shows the sale of products shall be paid for the service principle, to plans to sell the patent technology of the product market price and the market prospects, or network will according to the ministry of finance of intangible assets evaluation of relevant standards and regulations to give an objective and fair evaluation, specific charge standard, please consult customer service staff.

交易流程

根据中技网交易规则,技术买卖双方应当遵循友好、自愿和有偿原则进行交易,为此,请技术买卖双方应当遵循中技网制定的交易流程进行有序买卖。
一、买方确定购买意向→支付定金→签订意向性购买协议→买卖双方签订正式协议→买方付款 →办理技术转移交割手续→资金结算→交易完成→退还定金。

二、卖方提交信息资料→上传有效证明文件→平台审核→与平台签订相关协议和信用保证金协议→缴纳信用保证金→平台发布出让信息→确定买家→签订协议→出让技术→资金结算→交易完成→退还信用保证金。

    According to trading rules or network technology between buyer and seller shall follow the principle of friendly, voluntary and paid, for this, please technology between buyer and seller shall follow the wowed the orderly sale network of trading process.

One, the buyer to determine the purchase intentions to pay the deposit, intentional purchase agreement to sign a formal agreement between the seller and the buyer, the buyer's payment to delivery technology transfer formalities, fund settlement - deal - return deposit.

Second, the seller submit information to upload valid documents to review platform, and platform signed the relevant agreement and credit margin agreements to pay credit deposit, platform release transfer information to determine the buyer, signed an agreement to transfer technology, funds and settlements - deal - the refund credit deposit

重要提示

1、技术转让方请务必遵守《中华人民共和国合同法》、《中华人民共和国专利法》、《中华人民共和国知识产权法》、《中华人民共和国促进科技成果转化法》等相关法律法规,诚实守信,不得伪造或提供虚假技术信息。

2、技术购买方在决定购买技术商品前,首先要了解中技网的有关交易流程和技术转让方的详细信息,在购买过程中对有关技术信息产生疑问或不清楚的地方,请随时与技术信息发布单位取得联系。

3、中技网作为市场化运作的专业技术买卖服务平台,由中国技术市场协会主办,是国内权威的专业技术交易分类信息网,专业、专注、权威,面向国内外发布技术转让和技术需求项目信息,服务周到,信誉第一,交易规范,请客户认准中技网官方网址,切勿上当受骗。

1, technology transferor please be sure to abide by the law of the People's Republic of China contract law ", "patent law of the People's Republic of China" and "intellectual property law of the People's Republic of China", "promote the conversion of scientific and technological achievements of the People's Republic of China" and other relevant laws and regulations, the honest code of honor, are not allowed to fake or providing false information technology.

2 the purchaser prior to the decision to buy technology goods, technology, the first thing to understand the assignor or network related transaction process and technology of detailed information, in the process of buying to doubt the related technical information or unclear, please feel free to get in touch with technical information release unit.

3, or as a market-oriented operation of professional technology trading service platform, sponsored by the China technology market association, is the domestic authority of professional technology trading classified information network, professional, dedicated, authority, and the release technology transfer and technology at home and abroad demand project information, service, credit first, trade specification, please customers look or net official web site, please do not be deceived.

郑重声明

  会员在服务期内私自线下与相对交易方进行交易,由当事人自行承担法律后果,所产生的经济或知识产权纠纷中技网不承担任何法律责任。

Membership in the service period to offline, transacting business with relatively by the parties to bear the legal consequences, economic or intellectual property rights disputes or generated by the network does not assume any legal liability.

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